PUBLICATIONS AND PROJECTS
PUBLICATIONS
Barbalho, GN; Falcão, MA; Amaral, VA; Contarato, JLA; Cunha-Filho, M; Gelfuso, GM; Gratieri, T. Hydrogel-based hybrid membrane enhances in vitro drug evaluation in OphthalMimic device. Methods, Volume 230, October 2024, Pages 21-31 (https://doi.org/10.1016/j.ymeth.2024.07.010)
Barbalho, GN; Falcão, MA; Amaral, VA; Contarato, JLA; Cunha-Filho, M; Gelfuso, GM; Gratieri, T. OphthalMimic: a new alternative apparatus without animal tissue for the evaluation of topical ophthalmic drug products. Methods, Volume 228, August 2024, Pages 1-11 (https://doi.org/10.1016/j.ymeth.2024.05.005)
Barbalho, GN; Falcão, MA; Lopes, JMS; Lopes, JM; Contarato, JLA; Cunha-Filho, M; Gelfuso, GM; Gratieri, T. Dynamic Ex Vivo Porcine Eye Model to Measure Ophthalmic Drug Penetration under Simulated Lacrimal Flow. Pharmaceutics, v. 15(9), p. 2325, 2023. (https://doi.org/10.3390/pharmaceutics15092325)
Xavier, LC; Matos, BN; Barbalho, GN; Falcão, MA; Cunha-Filho, M; Gelfuso, GM; Gratieri, T. In Vitro Performance Analysis of a Minoxidil Thermosensitive Gel with Reduced Runoff for Eyebrow Hair Growth. Gels, v. 9(4), p. 269, 2023. (https://doi.org/10.3390/gels9040269)
Scopel, R; Falcão, MA; Cappellari, Ar; Morrone, FB; Guterres, SS; Cassel, E; Kasko, AM; Vargas, RMF. Lipid-polymer hybrid nanoparticles as a targeted drug delivery system for melanoma treatment. Int J Polym Mater, v. 71(2), p. 127-138, 2022. (https://doi.org/10.1080/00914037.2020.1809406)
Falcão,MA; Scopel, R; Almeida, RN; Do Espirito Santo, AT; Franceschini, G; Garcez, JJ; Vargas, RMF; Cassel, E. Supercritical fluid extraction of vinblastine from Catharanthus roseus. J Supercrit. Fluids, v. 129, p. 9-15, 2017. (https://doi.org/10.1016/j.supflu.2017.03.018)
Minteguiaga, M; Umpiérrez, N; Fariña, L; Falcão, MA.; Xavier, VB; Cassel, E; Dellacassa, E. Impact of gas chromatography and mass spectrometry combined with gas chromatography and olfactometry for the sex differentiation of by the analysis of volatile compounds. J. Sep. Sci. (Print), v. 38, p. 3038-3046, 2015. (https://doi.org/10.1002/jssc.201500131)
Scopel,R; Falcão, MA; Lucas, AM; Almeida, RN ; Gandolfi, PHK; Cassel, E; Vargas, RMF. Supercritical Fluid Extraction from Syzygium aromaticum Buds: Phase Equilibrium, Mathematical Modeling and Antimicrobial Activity. J Supercrit. Fluids, v. 92, p. 223-230, 2014. (https://doi.org/10.1016/j.supflu.2014.06.003)
Scopel, R; Neto, RG; Falcão, MA; Cassel, E; Vargas, RMF. Supercritical CO2 Extraction of Schinus molle L with Co-Solvents: Mathematical Modeling and Antimicrobial Applications. Braz Arch Biol Technol (Printed), v. 56, p. 513-519, 2013.
Vargas, RMF; Barroso, MST; Neto, RG; Scopel, R; Falcão, MA; Silva, CF; Cassel, E. Natural products obtained by subcritical and supercritical fluid extraction from Achyrocline satureioides (Lam) D.C. using CO2. Ind Crops Prod (Print), v. 50, p. 430-435, 2013.
Sartor, T.; Xavier, VB, Falcão, MA; Mondin, CA; Dos Santos, MA; Cassel, E; Astarita, LV; Santarém, ER. Seasonal changes in phenolic compounds and in the biological activities of Baccharis dentata (Vell.) G.M. Barroso. Ind Crops Prod (Print), v. 51, p. 355-359, 2013.
Fianco, ALB; Falcão, MA; Cassel, E; Milão, D. Determination of the antimicrobial activity and total polyphenol content of ethanolic extracts of propolis from stingless bees Tetragonisca angustula (Jataí) and Scaptotrigona bipunctata (Tubuna). Liberato Magazine (Novo Hamburgo), v. 14, p. 21-28, 20
Falcão, MA; Fianco, ALB; Lucas, AM; Pereira, MAA; Torres, FC Vargas, RMF; Cassel, E. Determination of antibacterial activity of vacuum distillation fractions of lemongrass essential oil. Phytochem Rev (Print), v. 11, p. 405-412, 2012.
INTELLECTUAL PROPERTIES
Falcão, MA; Barbalho, GN, Cunha-Filho, M; Gelfuso, GM; Gratieri, T.Hybrid Hydrogel-Based Films and its Use for In Vitro Evaluation of Ophthalmic Formulations Performance and Incorporating Reconstructed Human Cornea for Permeation Assessment. Intellectual Property - Patent, BR1020240146409, 2024
Falcão, MA; Cunha-Filho, M; Gelfuso, GM; Gratieri, T. Ophthalmic Performance Test Device Configuration. Intellectual Propriety - Industrial Design, BR3020230051546, 2023.
PROJECTS
Development of an in vitro model and device for ophthalmic drug product performance evaluation
(2022 - 2023)
Associated with Universidade de Brasília (UnB)
The regulatory requirements for ophthalmic formulations registration, generic or similar, oblige that the formulations have the same viscosity as a guarantee of the same performance. If the formulations do not have the same viscosity, which is very common, there are no in vitro methodologies to prove dynamic performance. Thus, this biotechnology project was created out of a need from the pharmaceutical industry demand. This project covers two completely innovative proposals, from its design and conception, of alternative methods to the use of animals for evaluating the performance of ophthalmic formulations during the research and development stages with a view to future use for regulatory purposes. They are the ex vivo Whole Eye Globe model with simulated tear flow and the OphthalMimic model, completely artificial for evaluating the dynamic performance of formulations. Initially, such models would be used in the research and development stages for comparisons between formulations, without an in vitro/in vivo correlation being possible.
Development of extraction processes and antineoplastic controlled release systems in supercritical environment
(2014-2016)
Associated with Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) and University of California, Los Angeles (UCLA)
Development of supercritical fluid extraction processes and antineoplastic controlled release systems, research based on the use of supercritical carbon dioxide in the pharmaceutical production chain, from the extraction of an antineoplastic drug (vinblastine) from plants to the processing and fabrication of polymeric films nanoimpregnated with therapeutic agents for controlled release. The project aimed to incorporate engineering knowledge into the processes of natural products supercritical fluids extraction, the production of polymeric nanosystems impregnated with antineoplastic drugs, and the study of in vitro controlled release kinetics.
Development of Biopesticides by plant extracts nanoimpregnation in biopolymers
(2012-2016)
Associated with Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
Optimization of biopesticides through the impregnation of bioactive botanical and/or fungal extracts in biopolymers. The extracts were selected from previous research projects results of the participating groups (LOPE – Brazil and CSIC – Spain). The selected biopolymers are modified natural polymers: chitosan cross-linked with glutaraldehyde, gelatin cross-linked with formaldehyde, and cellulose transformed into cellulose acetate.
Development of extraction processes and evaluation of the aromatic and medicinal potential of Mercosur native Baccharis species
(2012-2016)
Associated with Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) and Universidad de la Republica (UdelaR)
Development of South American native plant resources through the sustainable use of the genus Baccharis, with a focus on the search for extracts with commercialization potential. This project involves the systematic chemical study of plants of the genus Baccharis from the native flora identified at the Center for Research and Conservation of Nature Pró-Mata (CPCN Pró-Mata) of the Pontifícia Universidade Católica do Rio Grande do Sul, the technological development of productive processes, extracts natural products, rigorous quality control of the products obtained and a study of applications of the extracts obtained.
Modeling and Simulation of Essential Oils Process Production by Steam Distillation: Mass and Energy Balance
(2011-2016)
Associated with Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
Simulation and mathematical modeling of the steam distillation essential oil extraction process, using energy and mass balance with the objective of generating subsidies for a better relationship between the modeling and the results obtained in the industrial process. Since the mathematical simulation of an extractive process is a fundamental step for the implementation of processes at an industrial level with adequate and efficient characteristics, this project intends to estimate parameters from laboratory extractions (laboratory and pilot-scale), unavailable in the scientific literature.
Development of natural antimicrobial agents for use in body care products: essential oils and its fractions
(2010-2012)
Associated with Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
Study of the use of essential oils and their respective fractions obtained by vacuum distillation as natural antimicrobial agents for the development of body care products. Evaluate the influence of essential oils and their respective fractions composition on the antimicrobial activity against the microorganisms Salmonella choleraesuis, Pseudomonas aeruginosa, and Staphylococcus aureus, using qualitative and quantitative microbiological techniques. The essential oils selected for studies were lemongrass (Cymbopogon citratus), citronella (Cymbopogon winterianus) and Baccharis dracunculifolia.